1,3,7-trimethylxanthine Caffeine is stable at temperatures below 235°C (455°F), and few coffee roasters would roast a coffee past this temperature due to the risk of fire. So caffeine content remains pretty much constant throughout each stage of the roasting process. Q:Is a cup of dark roasted coffee has more caffeine? A cup of dark roast coffee will probably contain more caffeine than a cup of light roast. What? How? It’s all to do with the density of the coffee beans. As coffee is roasted darker and darker, the beans lose mass. But despite the loss of mass, the caffeine content stays the same. So it has higher caffeine content w/w, by ~ 5% to 9% If you brew a cup of coffee, whether it is a light or dark roasted, using same weight of roasted beans, the caffeine per cup will be slightly higher in a dark roasted coffee. The main reason that people think dark roast coffee has more caffeine is because of the increased bitterness and kick, which is associated with caffeine. It’s true...